Pla2
Pla2
EFEF
EF
In a rat
model of acute neuroinflammation, produced by a 6-day
intracerebral ventricular infusion of bacterial lipopolysaccharide (LPS), we measured brain activities and
protein levels of three phospholipases A2 (PLA2) and
of cyclo-oxygenase-1 and -2, and quantified other aspects of brain phospholipid and fatty acid metabolism. The 6-day intracerebral ventricular infusion increased lectin-reactive microglia in the
cerebral ventricles, pia mater, and the glial membrane of the cortex and resulted in morphological
changes of glial fibrillary
acidic protein (GFAP)-positive astrocytes in the
cortical mantel and areas surrounding the cerebral ventricles. LPS infusion
increased brain cytosolic and secretory
PLA2 activities by 71% and 47%, respectively, as well as the brain
concentrations of non-esterified linoleic
and arachidonic acids, and of prostaglandins E2 and
D2. LPS infusion also increased rates of incorporation and turnover of arachidonic acid in phosphatidylethanolamine,
plasmenylethanolamine, phosphatidylcholine,
and plasmenylcholine by 1.5- to 2.8-fold, without
changing these rates in phosphatidylserine or phosphatidylinositol. These observations suggest that
selective alterations in brain arachidonic acid
metabolism involving cytosolic and secretory PLA2 contribute to early pathology in neuroinflammation.
PMID: 15009672 [PubMed - in process]