Pclb
The membrane-bound
human liver UDP-glucuronosyltransferase UGT1*6 was
expressed in Escherichia coli. Exchange of the natural signal peptide by the
bacterial signal peptides of pclB or OmpT proteins considerably increased the level of
expression and, as the natural signal peptide, targeted the protein to the
membranes. The extent of maturation of SpelB-UGT1*6 precursor was about 30%. No
processing of sOmpT-UGT1*6 occurred but the processing rate of this precursor
could be significantly increased by mutagenesis of the first two amino acid
residues of the mature sequence. These expression vectors allowed us to produce
high levels of recombinant mature UGT1*6 required for further structural
studies.
PMID: 8313973 [PubMed - indexed for MEDLINE]