Desire :
Inflamm Res. 2007 Feb;56(2):70-5. Bacterial components regulate
the expression of Toll-like receptor 4 on human mast cells. ¡P
Kubo Y, Fukuishi
N, Yoshioka M,
Kawasoe Y,
Iriguchi
S, Imajo
N, Yasui
Y, Matsui N, Akagi
M. Department of Pharmacology, Faculty of
Pharmaceutical Sciences, Tokushima Bunri
University, Nishihama Bouji
180, Yamashiro-cho, Tokushima, 770-8514, Japan,
nobuf@ph.bunri-u.ac.jp. OBJECTIVES AND DESIGN: The aim of this study was to
investigate whether the exposure of mast cells (MCs) to bacterial
components affects the expression of Toll-like receptor (TLR) 4, and to
elucidate the behavior of MCs during the early response to infection.
MATERIALS: Two human MC lines, HMC-1 and LAD2, were employed. Messenger RNA
expression was observed by RT and real-time PCR. TLR4 expression was
determined by Western blotting. TNF-alpha secretion was analyzed with ELISA.
The degranulation ratio was measured with betahexosaminidase assay. RESULTS: Although bacterial
components increased TLR4 mRNA, only lipopolysaccharide
(LPS) augmented the TLR4 protein expression. LAD2 pre-treated with LPS for
8 h resulted in 2-fold increased TNF-alpha secretion on LPS restimulation. CONCLUSION: These results suggest that
the exposure of MCs to LPS may reinforce the innate immune system due to
up-regulation of MC TLR4, followed by increased TNF-alpha release. PMID: 17431743 [PubMed - in
process] J Ethnopharmacol.
2007 Feb 13; [Epub ahead of print] The inhibitory effect of Houttuynia cordata extract on
stem cell factor-induced HMC-1 cell migration. ¡P
Kim IS, Kim JH, Kim JS, Yun
CY, Kim DH, Lee JS. Department of Biomedical Laboratory Science,
School of Medicine, Eulji University, 143-5 Yeuongdu-dong, Jung-gu, Daejeon 301-832, Republic of Korea. Hottuynia cordata
Thunb (Saururaceae; HC)
is known as a therapeutic drug that has been used in traditional oriental
medicine for the treatment of allergy. Mast cells play an important role in
a variety of inflammatory diseases, and specifically asthma and atopy. In the present study, we investigated the effect
of HC extracts on the migration of the human mast cell line, HMC-1, in response to stem cell factor (SCF). Treatment with
HC extracts at a concentration of 10mug/ml for 24h showed no significant
decrease in the survival rate of the HMC-1 cells. SCF showed the typical
bell-shape curve for the HMC-1 cell chemoattraction
with the peak of the curve at the SCF concentration of 100ng/ml. HC-1,
which was the whole plant (Houttuynia cordata) extracted with 80% EtOH,
and HC-3, which was the residue successively partitioned with EtOAc, both had inhibitory effects on HMC-1 cell
movement. After the treatment with 10mug/ml HC-1 extract for 6 and 24h, the
chemotactic index (CI) of HMC-1 cells decreased
up to 74 and 63%, respectively. HC-3 extract treatment for 6 and 24h
lowered the CI to 72 and 44%, respectively. The HC-1 and HC-3 extracts had
no inhibitory effect on the mRNA and surface protein expressions of c-kit,
SCF receptor. SCF mediated the chemotaxis
signaling via NF-kappaB activation, and both
extracts inhibited the activation. Therefore, our results indicate that
HC-1 and HC-3 extracts decrease the chemotactic
ability of HMC-1 cells in response to SCF by inhibiting the NF-kappaB activation, and these substances may be useful
for treating mast cell-induced inflammatory diseases. PMID: 17368775 [PubMed - as
supplied by publisher]
