Flowchart: Preparation: HMC-1

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5/3/2007/-15          

Inflamm Res. 2007 Feb;56(2):70-5.Click here to read  Links

Bacterial components regulate the expression of Toll-like receptor 4 on human mast cells.

P     Kubo Y, Fukuishi N, Yoshioka M, Kawasoe Y, Iriguchi S, Imajo N, Yasui Y, Matsui N, Akagi M.

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Nishihama Bouji 180, Yamashiro-cho, Tokushima, 770-8514, Japan, nobuf@ph.bunri-u.ac.jp.

OBJECTIVES AND DESIGN: The aim of this study was to investigate whether the exposure of mast cells (MCs) to bacterial components affects the expression of Toll-like receptor (TLR) 4, and to elucidate the behavior of MCs during the early response to infection. MATERIALS: Two human MC lines, HMC-1 and LAD2, were employed. Messenger RNA expression was observed by RT and real-time PCR. TLR4 expression was determined by Western blotting. TNF-alpha secretion was analyzed with ELISA. The degranulation ratio was measured with betahexosaminidase assay. RESULTS: Although bacterial components increased TLR4 mRNA, only lipopolysaccharide (LPS) augmented the TLR4 protein expression. LAD2 pre-treated with LPS for 8 h resulted in 2-fold increased TNF-alpha secretion on LPS restimulation. CONCLUSION: These results suggest that the exposure of MCs to LPS may reinforce the innate immune system due to up-regulation of MC TLR4, followed by increased TNF-alpha release.

PMID: 17431743 [PubMed - in process]

J Ethnopharmacol. 2007 Feb 13; [Epub ahead of print]Click here to read  Links

The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration.

P     Kim IS, Kim JH, Kim JS, Yun CY, Kim DH, Lee JS.

Department of Biomedical Laboratory Science, School of Medicine, Eulji University, 143-5 Yeuongdu-dong, Jung-gu, Daejeon 301-832, Republic of Korea.

Hottuynia cordata Thunb (Saururaceae; HC) is known as a therapeutic drug that has been used in traditional oriental medicine for the treatment of allergy. Mast cells play an important role in a variety of inflammatory diseases, and specifically asthma and atopy. In the present study, we investigated the effect of HC extracts on the migration of the human mast cell line, HMC-1, in response to stem cell factor (SCF). Treatment with HC extracts at a concentration of 10mug/ml for 24h showed no significant decrease in the survival rate of the HMC-1 cells. SCF showed the typical bell-shape curve for the HMC-1 cell chemoattraction with the peak of the curve at the SCF concentration of 100ng/ml. HC-1, which was the whole plant (Houttuynia cordata) extracted with 80% EtOH, and HC-3, which was the residue successively partitioned with EtOAc, both had inhibitory effects on HMC-1 cell movement. After the treatment with 10mug/ml HC-1 extract for 6 and 24h, the chemotactic index (CI) of HMC-1 cells decreased up to 74 and 63%, respectively. HC-3 extract treatment for 6 and 24h lowered the CI to 72 and 44%, respectively. The HC-1 and HC-3 extracts had no inhibitory effect on the mRNA and surface protein expressions of c-kit, SCF receptor. SCF mediated the chemotaxis signaling via NF-kappaB activation, and both extracts inhibited the activation. Therefore, our results indicate that HC-1 and HC-3 extracts decrease the chemotactic ability of HMC-1 cells in response to SCF by inhibiting the NF-kappaB activation, and these substances may be useful for treating mast cell-induced inflammatory diseases.

PMID: 17368775 [PubMed - as supplied by publisher]