Aid
Brain tumor
Hiv
Ovarian
cancer
Gynecol Oncol. 2006 Apr
20; [Epub ahead of print] : Neurochem
Int. 2006 Apr 16; [Epub ahead of print]
Expression of chemokine
CXCL12 and its receptor CXCR4 in human epithelial ovarian cancer: An
independent prognostic factor for tumor progression.
Jiang YP, Wu
XH, Shi
B, Wu
WX, Yin
GR.
Department of Obstetrics and Gynecology, the
OBJECTIVES.: Chemokine
CXCL12 and its unique receptor CXCR4 have been recently implicated in
cancer metastasis. Our goal was to explore expression of CXCL12 and CXCR4
protein in normal ovarian surface epithelium, primary tumors and paired
metastases of epithelial ovarian cancer as well as its association with clinicopathological features. We also wanted to test if
expression of CXCR4 has prognostic value in epithelial ovarian cancer
patients. METHOD.: Sections from 6 normal ovarian
surface epithelium, 44 primary epithelial ovarian tumors and 30 paired metastatic tumors in omentum
were evaluated for CXCL12 and CXCR4 expression using immunohistochemistry
(IHC). RESULTS.: All samples of normal ovarian
surface epithelium were negative for CXCL12 and CXCR4 protein. Ovarian
cancer cells mainly showed cytoplasmic staining
of CXCL12 and CXCR4. CXCL12 and CXCR4 staining were detected in 40/44 (91%)
and 26/44 (59%) patients with primary epithelial ovarian tumors
respectively. CXCR4 expression in primary tumors had no significant
correlation with lymph nodes metastasis. However, if we combined CXCR4
expression in primary tumors with metastatic
tumors, a significant correlation with lymph nodes metastasis was found (P
= 0.018). The intensity of CXCL12 staining correlated with ascites (P = 0.014). The rate of CXCR4 expression in
refractory and recurrent group (81% versus 28%, P = 0.0008) was
significantly higher than that in no-recurrent group. After a median
follow-up of 37 months, CXCR4 expression was found associated with an
unfavorable prognosis with significantly reduced median disease
progression-free survival and overall survival of 15 and 27 months (P =
0.0004, P = 0.017) respectively. Median time-to-event was not reached in
patients with negative CXCR4 staining. In multivariate analysis, CXCR4
expression and residual tumor size emerged as independent prognostic
factors in epithelial ovarian cancer patients. CONCLUSIONS.:
This article provides the first evidence that CXCR4 expression could be an
independent prognostic factor for epithelial ovarian cancer patients.
PMID: 16631235 [PubMed - as supplied by
publisher]
Expression of CXC chemokine
receptors 1-5 and their ligands in human glioma tissues: Role of CXCR4 and SDF1 in glioma cell proliferation and migration.
Bajetto A, Barbieri F, Dorcaratto A, Barbero S, Daga A, Porcile C, Ravetti JL, Zona G, Spaziante R, Corte
G, Schettini G, Florio T.
Department of Oncology, Biology and Genetics,
Chemokines have been involved in cellular
processes associated to malignant transformation such as proliferation,
migration and angiogenesis. The expression of five CXC chemokine
receptors and their main ligands was analysed by RT-PCR in 31 human astrocytic
neoplasms. The mRNAs for all the receptors analysed were identified in a high percentage of tumours, while their ligands
showed lower expression. CXCR4 and SDF1 were the most frequently mRNA
identified (29/31 and 13/31 of the gliomas
studied, respectively). Thus, we further analysed
the cell localization of CXCR4 and SDF1 in immunohistochemistry
experiments. We show a marked co-localization of CXCR4 and SDF1 in tumour cells, mainly evident in psudolpalisade
and microcystic degeneration areas and in the
vascular endothelium. In addition, hSDF1alpha induced a significant
increase of DNA synthesis in primary human glioblastoma
cell cultures and chemotaxis in a glioblastoma cell line. These results provide evidence
of the expression of multiple CXC chemokines and
their receptors in brain tumours and that in
particular CXCR4 and SDF1 sustain proliferation and migration of glioma cells to promote malignant progression.
PMID: 16621164 [PubMed - as supplied by
publisher]