Caspase3
AIM: To study the effect of NF-kappaB, survivin, Bcl-2 and
Caspase3 on tumor necrosis factors related apoptosis inducing ligand (TRAIL)
induced apoptosis of gastric cancer cells. METHODS: Gastric cancer cells of
SGC-7901, MKN28, MKN45 and AGS lines were cultured in PRMI-1640 medium and the
apoptosis rates of the cells of 4 lines were observed after treatment of tumor
necrosis factors related apoptosis inducing ligand (TRAIL) with a flow
cytometer. The expression of NF-kappaB, survivin, Bcl-2 and Caspase3 in gastric
cancer cells of 4 lines was analyzed with Western blot. RESULTS: After the
gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the
apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and
SGC-7901cells respectively. Western blot revealed that the expressions of
NF-kappaB and survivin were lower in MKN28 cells than in MKN45, AGS and
SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28
cells than in MKN45, AGS and SGC-7901 cells. CONCLUSION: There is a selectivity
of TRAIL potency to induce apoptosis in gastric cancer cells of different cell
lines. The anticancer potency of TRAIL is associated with the decreased
expression of NF-kappaB and survivin and increased expression of Caspase3 of
gastric cancer cells.
PMID: 14695762 [PubMed - in process]
